Biological Control of Gom-chwi (Ligularia fischeri) Phytophthora Root Rot with Enterobacter asburiae ObRS-5 to Suppress Zoosporangia Formation and Zoospores Germination.
Identifieur interne : 000282 ( Main/Exploration ); précédent : 000281; suivant : 000283Biological Control of Gom-chwi (Ligularia fischeri) Phytophthora Root Rot with Enterobacter asburiae ObRS-5 to Suppress Zoosporangia Formation and Zoospores Germination.
Auteurs : Kim [Corée du Sud] ; Sang Yeob Lee [Corée du Sud] ; Seong Ho Ahn [Corée du Sud] ; Ji Hee Han [Corée du Sud] ; Jin Woo Park [Corée du Sud]Source :
- The plant pathology journal [ 1598-2254 ] ; 2020.
Abstract
Gom-chwi (Ligularia fischeri) is severely infected with Phytophthora drechsleri, the causal organism of Phytophthora root rot, an economically important crop disease that needs management throughout the cultivation period. In the present study, Phytophthora root rot was controlled by using bacterial isolates from rhizosphere soils collected from various plants and screened for antagonistic activity against P. drechsleri. A total of 172 bacterial strains were isolated, of which, 49 strains showed antagonistic activities by dual culture assay. In the seedling assay, six out of the 49 strains showed a predominant effect on suppressing P. drechsleri. Among the six strains, the ObRS-5 strain showed remarkable against P. drechsleri when treated with seed dipping or soil drenching. The ObRS-5 strain was identified as Enterobacter asburiae based on 16S ribosomal RNA gene sequences analysis. The bacterial cells of E. asburiae ObRS-5 significantly suppressed sporangium formation and zoospore germination in P. drechsleri by 87.4% and 66.7%, respectively. In addition, culture filtrate of E. asburiae ObRS-5 also significantly inhibited sporangium formation and zoospore germination by 97.0% and 67.6%, respectively. Soil drenched bacterial cells, filtrate, and culture solution of E. asburiae ObRS-5 effectively suppressed Phytophthora root rot by 63.2%, 57.9%, and 81.1%, respectively. Thus, E. asburiae ObRS-5 could be used as a potential agent for the biological control of Phytophthora root rot infecting gom-chwi.
DOI: 10.5423/PPJ.OA.11.2019.0283
PubMed: 32547340
PubMed Central: PMC7272845
Affiliations:
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Agricultural Microbiology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Agricultural Microbiology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365</wicri:regionArea><wicri:noRegion>Wanju 55365</wicri:noRegion></affiliation></author><author><name sortKey="Ahn, Seong Ho" sort="Ahn, Seong Ho" uniqKey="Ahn S" first="Seong Ho" last="Ahn">Seong Ho Ahn</name><affiliation wicri:level="1"><nlm:affiliation>Division of Agricultural Microbiology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Agricultural Microbiology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365</wicri:regionArea><wicri:noRegion>Wanju 55365</wicri:noRegion></affiliation></author><author><name sortKey="Han, Ji Hee" sort="Han, Ji Hee" uniqKey="Han J" first="Ji Hee" last="Han">Ji Hee Han</name><affiliation wicri:level="1"><nlm:affiliation>Division of Agricultural Microbiology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Agricultural Microbiology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365</wicri:regionArea><wicri:noRegion>Wanju 55365</wicri:noRegion></affiliation></author><author><name sortKey="Park, Jin Woo" sort="Park, Jin Woo" uniqKey="Park J" first="Jin Woo" last="Park">Jin Woo Park</name><affiliation wicri:level="1"><nlm:affiliation>Division of Agricultural Microbiology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Korea.</nlm:affiliation><country xml:lang="fr">Corée du 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with <i>Enterobacter asburiae</i> ObRS-5 to Suppress Zoosporangia Formation and Zoospores Germination.</title><author><name sortKey="Kim" sort="Kim" uniqKey="Kim" last="Kim">Kim</name><affiliation wicri:level="1"><nlm:affiliation>Division of Agricultural Microbiology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Agricultural Microbiology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365</wicri:regionArea><wicri:noRegion>Wanju 55365</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Sang Yeob" sort="Lee, Sang Yeob" uniqKey="Lee S" first="Sang Yeob" last="Lee">Sang Yeob Lee</name><affiliation wicri:level="1"><nlm:affiliation>Division of Agricultural Microbiology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Agricultural Microbiology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365</wicri:regionArea><wicri:noRegion>Wanju 55365</wicri:noRegion></affiliation></author><author><name sortKey="Ahn, Seong Ho" sort="Ahn, Seong Ho" uniqKey="Ahn S" first="Seong Ho" last="Ahn">Seong Ho Ahn</name><affiliation wicri:level="1"><nlm:affiliation>Division of Agricultural Microbiology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Agricultural Microbiology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365</wicri:regionArea><wicri:noRegion>Wanju 55365</wicri:noRegion></affiliation></author><author><name sortKey="Han, Ji Hee" sort="Han, Ji Hee" uniqKey="Han J" first="Ji Hee" last="Han">Ji Hee Han</name><affiliation wicri:level="1"><nlm:affiliation>Division of Agricultural Microbiology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Agricultural Microbiology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365</wicri:regionArea><wicri:noRegion>Wanju 55365</wicri:noRegion></affiliation></author><author><name sortKey="Park, Jin Woo" sort="Park, Jin Woo" uniqKey="Park J" first="Jin Woo" last="Park">Jin Woo Park</name><affiliation wicri:level="1"><nlm:affiliation>Division of Agricultural Microbiology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Division of Agricultural Microbiology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365</wicri:regionArea><wicri:noRegion>Wanju 55365</wicri:noRegion></affiliation></author></analytic><series><title level="j">The plant pathology journal</title><idno type="ISSN">1598-2254</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Gom-chwi (<i>Ligularia fischeri</i>) is severely infected with <i>Phytophthora drechsleri</i>, the causal organism of Phytophthora root rot, an economically important crop disease that needs management throughout the cultivation period. In the present study, Phytophthora root rot was controlled by using bacterial isolates from rhizosphere soils collected from various plants and screened for antagonistic activity against <i>P. drechsleri</i>. A total of 172 bacterial strains were isolated, of which, 49 strains showed antagonistic activities by dual culture assay. In the seedling assay, six out of the 49 strains showed a predominant effect on suppressing <i>P. drechsleri</i>. Among the six strains, the ObRS-5 strain showed remarkable against <i>P. drechsleri</i> when treated with seed dipping or soil drenching. The ObRS-5 strain was identified as <i>Enterobacter asburiae</i> based on 16S ribosomal RNA gene sequences analysis. The bacterial cells of <i>E. asburiae</i> ObRS-5 significantly suppressed sporangium formation and zoospore germination in <i>P. drechsleri</i> by 87.4% and 66.7%, respectively. In addition, culture filtrate of <i>E. asburiae</i> ObRS-5 also significantly inhibited sporangium formation and zoospore germination by 97.0% and 67.6%, respectively. Soil drenched bacterial cells, filtrate, and culture solution of <i>E. asburiae</i> ObRS-5 effectively suppressed Phytophthora root rot by 63.2%, 57.9%, and 81.1%, respectively. Thus, <i>E. asburiae</i> ObRS-5 could be used as a potential agent for the biological control of Phytophthora root rot infecting gom-chwi.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">32547340</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>28</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1598-2254</ISSN><JournalIssue CitedMedium="Print"><Volume>36</Volume><Issue>3</Issue><PubDate><Year>2020</Year><Month>Jun</Month><Day>01</Day></PubDate></JournalIssue><Title>The plant pathology journal</Title><ISOAbbreviation>Plant Pathol J</ISOAbbreviation></Journal><ArticleTitle>Biological Control of Gom-chwi (<i>Ligularia fischeri</i>) Phytophthora Root Rot with <i>Enterobacter asburiae</i> ObRS-5 to Suppress Zoosporangia Formation and Zoospores Germination.</ArticleTitle><Pagination><MedlinePgn>244-254</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.5423/PPJ.OA.11.2019.0283</ELocationID><Abstract><AbstractText>Gom-chwi (<i>Ligularia fischeri</i>) is severely infected with <i>Phytophthora drechsleri</i>, the causal organism of Phytophthora root rot, an economically important crop disease that needs management throughout the cultivation period. In the present study, Phytophthora root rot was controlled by using bacterial isolates from rhizosphere soils collected from various plants and screened for antagonistic activity against <i>P. drechsleri</i>. A total of 172 bacterial strains were isolated, of which, 49 strains showed antagonistic activities by dual culture assay. In the seedling assay, six out of the 49 strains showed a predominant effect on suppressing <i>P. drechsleri</i>. Among the six strains, the ObRS-5 strain showed remarkable against <i>P. drechsleri</i> when treated with seed dipping or soil drenching. The ObRS-5 strain was identified as <i>Enterobacter asburiae</i> based on 16S ribosomal RNA gene sequences analysis. The bacterial cells of <i>E. asburiae</i> ObRS-5 significantly suppressed sporangium formation and zoospore germination in <i>P. drechsleri</i> by 87.4% and 66.7%, respectively. In addition, culture filtrate of <i>E. asburiae</i> ObRS-5 also significantly inhibited sporangium formation and zoospore germination by 97.0% and 67.6%, respectively. Soil drenched bacterial cells, filtrate, and culture solution of <i>E. asburiae</i> ObRS-5 effectively suppressed Phytophthora root rot by 63.2%, 57.9%, and 81.1%, respectively. Thus, <i>E. asburiae</i> ObRS-5 could be used as a potential agent for the biological control of Phytophthora root rot infecting gom-chwi.</AbstractText><CopyrightInformation>© The Korean Society of Plant Pathology.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kim</LastName><AffiliationInfo><Affiliation>Division of Agricultural Microbiology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Sang Yeob</ForeName><Initials>SY</Initials><AffiliationInfo><Affiliation>Division of Agricultural Microbiology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ahn</LastName><ForeName>Seong Ho</ForeName><Initials>SH</Initials><AffiliationInfo><Affiliation>Division of Agricultural Microbiology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Han</LastName><ForeName>Ji Hee</ForeName><Initials>JH</Initials><AffiliationInfo><Affiliation>Division of Agricultural Microbiology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Park</LastName><ForeName>Jin Woo</ForeName><Initials>JW</Initials><AffiliationInfo><Affiliation>Division of Agricultural Microbiology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Korea.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Korea (South)</Country><MedlineTA>Plant Pathol J</MedlineTA><NlmUniqueID>100966769</NlmUniqueID><ISSNLinking>1598-2254</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Enterobacter asburiae ObRS-5</Keyword><Keyword MajorTopicYN="N">Ligularia fischeri</Keyword><Keyword MajorTopicYN="N">Phytophthora drechsleri</Keyword><Keyword MajorTopicYN="N">antagonism</Keyword><Keyword MajorTopicYN="N">biological control</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>11</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>03</Month><Day>31</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>04</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>6</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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IdType="pubmed">24885352</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Corée du Sud</li></country></list><tree><country name="Corée du Sud"><noRegion><name sortKey="Kim" sort="Kim" uniqKey="Kim" last="Kim">Kim</name></noRegion><name sortKey="Ahn, Seong Ho" sort="Ahn, Seong Ho" uniqKey="Ahn S" first="Seong Ho" last="Ahn">Seong Ho Ahn</name><name sortKey="Han, Ji Hee" sort="Han, Ji Hee" uniqKey="Han J" first="Ji Hee" last="Han">Ji Hee Han</name><name sortKey="Lee, Sang Yeob" sort="Lee, Sang Yeob" uniqKey="Lee S" first="Sang Yeob" last="Lee">Sang Yeob Lee</name><name sortKey="Park, Jin Woo" sort="Park, Jin Woo" uniqKey="Park J" first="Jin Woo" last="Park">Jin Woo Park</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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